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c16 ceramide (43799)  (Millipore)

 
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    Millipore c16 ceramide (43799)
    Ligand activity of TAG-related molecules and other endogenous lipids. Siglec5 reporter cells were stimulated with plate-coated lipids for 18 h. Induction of NFAT was analyzed by flow cytometry. A, C18 TAGs with saturated or unsaturated fatty acid chains. Chemical structures are shown in Fig. S3C. B, glycerol and C18 saturated or unsaturated fatty acids. C, C18:2 DAG and MAG. Chemical structures are shown in Fig. S3D. D, glycerophospholipids, such as phosphatidylcholine (PC), PE, phosphatidylserine (PS), phosphatidylinositol (PI), and PG. E, cardiolipin. F, <t>C16</t> <t>ceramide</t> and sphingomyelin. G, cholesterol and cholesterol ester (cholesteryl linoleate). H, 5-PAHSA and 9-PAHSA. Chemical structures are shown in Fig. S3E. I, recombinant Siglec5-Fc and Fc proteins were incubated with plate-coated cardiolipin or 5-PAHSA. Bound proteins were detected with anti-hIgG-HRP. Data are representative of at least three independent experiments and are presented as the mean ± S.D. (error bars). *, p < 0.05; **, p < 0.01.
    C16 Ceramide (43799), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c16 ceramide (43799)/product/Millipore
    Average 90 stars, based on 1 article reviews
    c16 ceramide (43799) - by Bioz Stars, 2026-02
    90/100 stars

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    1) Product Images from "Identification of lipophilic ligands of Siglec5 and -14 that modulate innate immune responses"

    Article Title: Identification of lipophilic ligands of Siglec5 and -14 that modulate innate immune responses

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.RA119.009835

    Ligand activity of TAG-related molecules and other endogenous lipids. Siglec5 reporter cells were stimulated with plate-coated lipids for 18 h. Induction of NFAT was analyzed by flow cytometry. A, C18 TAGs with saturated or unsaturated fatty acid chains. Chemical structures are shown in Fig. S3C. B, glycerol and C18 saturated or unsaturated fatty acids. C, C18:2 DAG and MAG. Chemical structures are shown in Fig. S3D. D, glycerophospholipids, such as phosphatidylcholine (PC), PE, phosphatidylserine (PS), phosphatidylinositol (PI), and PG. E, cardiolipin. F, C16 ceramide and sphingomyelin. G, cholesterol and cholesterol ester (cholesteryl linoleate). H, 5-PAHSA and 9-PAHSA. Chemical structures are shown in Fig. S3E. I, recombinant Siglec5-Fc and Fc proteins were incubated with plate-coated cardiolipin or 5-PAHSA. Bound proteins were detected with anti-hIgG-HRP. Data are representative of at least three independent experiments and are presented as the mean ± S.D. (error bars). *, p < 0.05; **, p < 0.01.
    Figure Legend Snippet: Ligand activity of TAG-related molecules and other endogenous lipids. Siglec5 reporter cells were stimulated with plate-coated lipids for 18 h. Induction of NFAT was analyzed by flow cytometry. A, C18 TAGs with saturated or unsaturated fatty acid chains. Chemical structures are shown in Fig. S3C. B, glycerol and C18 saturated or unsaturated fatty acids. C, C18:2 DAG and MAG. Chemical structures are shown in Fig. S3D. D, glycerophospholipids, such as phosphatidylcholine (PC), PE, phosphatidylserine (PS), phosphatidylinositol (PI), and PG. E, cardiolipin. F, C16 ceramide and sphingomyelin. G, cholesterol and cholesterol ester (cholesteryl linoleate). H, 5-PAHSA and 9-PAHSA. Chemical structures are shown in Fig. S3E. I, recombinant Siglec5-Fc and Fc proteins were incubated with plate-coated cardiolipin or 5-PAHSA. Bound proteins were detected with anti-hIgG-HRP. Data are representative of at least three independent experiments and are presented as the mean ± S.D. (error bars). *, p < 0.05; **, p < 0.01.

    Techniques Used: Activity Assay, Flow Cytometry, Recombinant, Incubation



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    c16 ceramide (43799)  (Millipore)
    90
    Millipore c16 ceramide (43799)
    Ligand activity of TAG-related molecules and other endogenous lipids. Siglec5 reporter cells were stimulated with plate-coated lipids for 18 h. Induction of NFAT was analyzed by flow cytometry. A, C18 TAGs with saturated or unsaturated fatty acid chains. Chemical structures are shown in Fig. S3C. B, glycerol and C18 saturated or unsaturated fatty acids. C, C18:2 DAG and MAG. Chemical structures are shown in Fig. S3D. D, glycerophospholipids, such as phosphatidylcholine (PC), PE, phosphatidylserine (PS), phosphatidylinositol (PI), and PG. E, cardiolipin. F, <t>C16</t> <t>ceramide</t> and sphingomyelin. G, cholesterol and cholesterol ester (cholesteryl linoleate). H, 5-PAHSA and 9-PAHSA. Chemical structures are shown in Fig. S3E. I, recombinant Siglec5-Fc and Fc proteins were incubated with plate-coated cardiolipin or 5-PAHSA. Bound proteins were detected with anti-hIgG-HRP. Data are representative of at least three independent experiments and are presented as the mean ± S.D. (error bars). *, p < 0.05; **, p < 0.01.
    C16 Ceramide (43799), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c16 ceramide (43799)/product/Millipore
    Average 90 stars, based on 1 article reviews
    c16 ceramide (43799) - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Ligand activity of TAG-related molecules and other endogenous lipids. Siglec5 reporter cells were stimulated with plate-coated lipids for 18 h. Induction of NFAT was analyzed by flow cytometry. A, C18 TAGs with saturated or unsaturated fatty acid chains. Chemical structures are shown in Fig. S3C. B, glycerol and C18 saturated or unsaturated fatty acids. C, C18:2 DAG and MAG. Chemical structures are shown in Fig. S3D. D, glycerophospholipids, such as phosphatidylcholine (PC), PE, phosphatidylserine (PS), phosphatidylinositol (PI), and PG. E, cardiolipin. F, C16 ceramide and sphingomyelin. G, cholesterol and cholesterol ester (cholesteryl linoleate). H, 5-PAHSA and 9-PAHSA. Chemical structures are shown in Fig. S3E. I, recombinant Siglec5-Fc and Fc proteins were incubated with plate-coated cardiolipin or 5-PAHSA. Bound proteins were detected with anti-hIgG-HRP. Data are representative of at least three independent experiments and are presented as the mean ± S.D. (error bars). *, p < 0.05; **, p < 0.01.

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of lipophilic ligands of Siglec5 and -14 that modulate innate immune responses

    doi: 10.1074/jbc.RA119.009835

    Figure Lengend Snippet: Ligand activity of TAG-related molecules and other endogenous lipids. Siglec5 reporter cells were stimulated with plate-coated lipids for 18 h. Induction of NFAT was analyzed by flow cytometry. A, C18 TAGs with saturated or unsaturated fatty acid chains. Chemical structures are shown in Fig. S3C. B, glycerol and C18 saturated or unsaturated fatty acids. C, C18:2 DAG and MAG. Chemical structures are shown in Fig. S3D. D, glycerophospholipids, such as phosphatidylcholine (PC), PE, phosphatidylserine (PS), phosphatidylinositol (PI), and PG. E, cardiolipin. F, C16 ceramide and sphingomyelin. G, cholesterol and cholesterol ester (cholesteryl linoleate). H, 5-PAHSA and 9-PAHSA. Chemical structures are shown in Fig. S3E. I, recombinant Siglec5-Fc and Fc proteins were incubated with plate-coated cardiolipin or 5-PAHSA. Bound proteins were detected with anti-hIgG-HRP. Data are representative of at least three independent experiments and are presented as the mean ± S.D. (error bars). *, p < 0.05; **, p < 0.01.

    Article Snippet: Tetradecane (172456), pentadecane (P3406), octadecane (O0652), eicosane (219274), docosane (134457), tetracosane (T8752), squalane (234311), cardiolipin (C1649), C16 ceramide (43799), sphingomyelin (S0756), cholesterol (C8667), cholesteryl linoleate (C0289), and linoleic acid (L1376) were purchased from Sigma-Aldrich.

    Techniques: Activity Assay, Flow Cytometry, Recombinant, Incubation